Personnel Information

写真b

NISHIMURA KENJI

Organization
School of Biological and Environmental Sciences
Research Fields, Keywords
オルガネラ, タンパク質分解, プロテアーゼ, 葉緑体
Teaching and Research Fields
葉緑体は光合成をはじめとする植物の正常な発達や成長に欠かせない多くの代謝反応が行われる細胞内小器官(オルガネラ)です。葉緑体内には延べ数千種類にも及ぶタンパク質が存在し、これら生体反応を触媒しています。そのため植物はこれら葉緑体タンパク質の活性や機能を維持・制御することにより、葉緑体代謝を円滑に進める必要があります。特に高次構造の形成異常や損傷などにより機能不全を起こしたタンパク質や、細胞内外の環境変化によりそれ自体が不要になった場合は、分解除去される必要があります。この役割を担うのが葉緑体タンパク質分解酵素(プロテアーゼ)です。葉緑体内にはこれまでのところ少なくとも20種類のプロテアーゼが存在することが知られています。我々はこれら葉緑体プロテアーゼのうち、どれがいつどこでどのタンパク質をなぜどのように分解しているかについて興味を持っています。また葉緑体タンパク質分解がどのような細胞内の現象と結びついているのか、さらに他のオルガネラを含む細胞内環境の変化と葉緑体タンパク質の恒常性との関連性についても明らかにしたいと考えています。

Degree 【 display / non-display

  • Degree name:博士(バイオサイエンス)
    Classified degree field:Life Science / Plant molecular biology and physiology
    Conferring institution:Nara Institute of Science and Technology
    Acquisition way:Coursework
    Date of acquisition:2010.09

Research Areas 【 display / non-display

  • Research field:Life Science / Plant molecular biology and physiology

Papers 【 display / non-display

  • Language: English
    Title: Genetic analysis of chlorophyll synthesis and degradation regulated by BALANCE of CHLOROPHYLL METABOLISM.
    Journal name: Plant physiology  vol.189  (1)  (p.419 - 432)
    Date of publication: 2022.03
    Author(s): Hiroshi Yamatani, Takeshi Ito, Kenji Nishimura, Tetsuya Yamada, Wataru Sakamoto, Makoto Kusaba

    DOI: 10.1093/plphys/kiac059
    Type of publication: Research paper (scientific journal)

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  • Language: English
    Title: Deciphering the Proteotoxic Stress Responses Triggered by the Perturbed Thylakoid Proteostasis in Arabidopsis.
    Journal name: Plants (Basel, Switzerland)  vol.10  (3)
    Date of publication: 2021.03
    Author(s): Kenji Nishimura, Reiko Nakagawa, Chisato Hachisuga, Yuri Nakajima Munekage

    DOI: 10.3390/plants10030519
    Type of publication: Research paper (scientific journal)

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  • Title: 葉緑体プロテアーゼによるオルガネラ恒常性と細胞内情報伝達の制御
    Journal name: BSJ-Review  vol.12  (A)  (p.52 - 67)
    Date of publication: 2021
    Author(s): 西村 健司

    DOI: 10.24480/bsj-review.12a7.00200

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  • Language: English
    Title: Posttranslational Control of ALA Synthesis Includes GluTR Degradation by Clp Protease and Stabilization by GluTR-Binding Protein
    Journal name: Plant Physiology
    Date of publication: 2016.03
    Author(s): Janina Apitz, Kenji Nishimura, Judith Schmied, Anja Wolf, Boris Hedtke, Klaas J. van Wijk, Bernhard Grimm

    Type of publication: Research paper (scientific journal)
    Co-author classification: Multiple Authorship

  • Language: English
    Title: Discovery of a Unique Clp Component, ClpF, in Chloroplasts: A Proposed Binary ClpF-ClpS1 Adaptor Complex Functions in Substrate Recognition and Delivery.
    Journal name: The Plant Cell
    Date of publication: 2015.11
    Author(s): Kenji Nishimura, Janina Apitz, Giulia Friso, Jitae Kim, Lalit Ponnala, Bernhard Grimm, Klaas J. van Wijk

    Type of publication: Research paper (scientific journal)
    Co-author classification: Multiple Authorship

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MISC 【 display / non-display

  • Language:English
    Title:Essentials of Proteolytic Machineries in Chloroplasts
    Journal name:Molecular Plant
    Date of publication:2017.01
    Author(s):KenjiNishimuraYusukeKatoWataruSakamoto

    Type of publication:Article, review, commentary, editorial, etc. (scientific journal)

  • Language:English
    Title:Chloroplast Proteases: Updates on Proteolysis within and across Suborganellar Compartments
    Journal name:Plant Physiology
    Date of publication:2016.08
    Author(s):Kenji Nishimura, Yusuke Kato, Wataru Sakamoto

    Type of publication:Article, review, commentary, editorial, etc. (scientific journal)

  • Language:English
    Title:Organization, function and substrates of the essential Clp protease system in plastids
    Journal name:Biochimica et Biophysica Acta Bioenergetics
    Date of publication:2014.12
    Author(s):Kenji Nishimura, Klaas J.van Wijk

    Type of publication:Article, review, commentary, editorial, etc. (scientific journal)

Research Projects 【 display / non-display

  • Project year:2022.04 - 2023.03
    Title:葉緑体内膜系界面を反応場とする新たなストレス応答の分子基盤
    Awarding organization:公益財団法人木下記念事業団
    System name:木下基礎科学研究基金助成事業

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  • Research category:Grant-in-Aid for Research Activity start-up
    Project year:2019.08 - 2021.03
    Title:葉緑体タンパク質由来ペプチドによるシグナル伝達機構の解明

  • Research category:Grant-in-Aid for Research Activity Start-up
    Project year:2019.08 - 2021.03
    Title:Resolving a possible signaling mechanism involving proteolytic peptides from chloroplasts
    Project summary:Protein degradation in particular processive proteolysis may be considered as a process of repurposing unwanted or dysfunctional proteins for generating functional molecules namely proteolytic peptides. This study investigated the fate and function of the peptides generated through proteolysis within chloroplasts. The results may suggest that peptides generated by chloroplast processive protease might be recognized by a plant immune receptor protein and that peptide release from chloroplasts could influence nuclear transcriptome.
    Awarding organization:Japan Society for the Promotion of Science
    System name:Grants-in-Aid for Scientific Research Grant-in-Aid for Research Activity Start-up

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