Personnel Information

写真b

FUJIWARA Shinsuke

Organization
School of Biological and Environmental Sciences Department of Biosciences Professor
Research Fields, Keywords
Polyamine microbiology archaea hyperthermophiles, 特殊環境微生物学, 生物工学, Polyamine microbiology archaea hyperthermophiles , 微生物生化学, 特殊環境微生物学, 生物工学
Teaching and Research Fields
地球上には、多様な環境に様々な微生物が生育している。90℃を超える温度を好んで生育する微生物を超好熱菌というが、その多くは温泉や、海底熱水鉱床など原始地球を思わせる環境に生育し、原始生命に最も近い現存生物として注目されている。超好熱菌の中でもArchaeaの分類ドメインに属するものは形態的には原核生物であるが、分子系統的に真核生物に近縁であり、真核細胞誕生の謎を探る上でも興味深い。しかも超好熱菌の生産する酵素は100℃を越える温度でも変性しない耐熱性酵素であり、産業上の用途が広い。私は超好熱菌の分子育種に取り組みつつ、耐熱性酵素の有効利用法を開発していきたいと思っている。遺伝子操作を駆使して酵素の機能改変を行いつつ、一方で有機溶媒などの疎水系で耐熱性酵素を機能させることで従来にない酵素反応場の実現を目指したい。また、新規有用微生物の探索や微生物を用いた環境浄化にも取り組み、微生物を利用したバイオテクノロジーを展開して行きたいと考えている。また、日本は発酵食品の宝庫である。発酵食品にはポリアミンなど健康に関係する物質が多く含まれる。麹菌、納豆菌、酢酸菌を中心に発酵食品に関与する微生物の機能を高め、食べ物から健康に貢献したいと考えている。
Syllabus information URL
https://syllabus.kwansei.ac.jp/uniasv2/UnSSOLoginControlFree?REQ_ACTION_DO=/AGA030PLS01Action.do?REQ_FUNCTION_JUMP_START_FLG=1&SLB_LINK_DISP_FLG=26&TCH_NO=020090&REQ_PRFR_FUNC_ID=AGA030
SDGs Related Goals
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Graduating School 【 display / non-display 】

  • Graduating School:Hiroshima University
    Faculty:Graduate School, Division of Biophere Sciences

    Kind of school:University
    Date of graduation:1990
    Completion status:Graduated

  • Graduating School:Hiroshima University
    Faculty:生物圏科学研究科
    Course / Major:生物機能科学

    Kind of school:University
    Date of graduation:1990
    Completion status:Graduated
    Country location code:Japan

  • Graduating School:Hiroshima University
    Faculty:Faculty of Applied Biological Science

    Kind of school:University
    Date of graduation:1985
    Completion status:Graduated

  • Graduating School:Hiroshima University
    Faculty:Faculty of Applied Biological Science
    Course / Major:生物生産学科

    Kind of school:University
    Date of graduation:1985
    Completion status:Graduated
    Country location code:Japan

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Graduate School 【 display / non-display 】

  • Graduate school:Hiroshima University
    Department:Graduate School, Division of Biophere Sciences
    Course:生物機能科学専攻

    Course completed:Doctor's Course
    Date of completion:1990.03
    Completion status:Accomplished credits for doctoral program
    Country:Japan

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Degree 【 display / non-display 】

  • Degree name:学術博士
    Classified degree field:Life Science / Applied microbiology
    Conferring institution:Hiroshima University
    Acquisition way:Thesis
    Date of acquisition:1991.02

  • Degree name:学術修士
    Classified degree field:Life Science / Applied microbiology
    Conferring institution:Hiroshima University
    Acquisition way:Coursework
    Date of acquisition:1987.03

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Career 【 display / non-display 】

  • Affiliation:Kwansei Gakuin University
    Title:Professor
    Date:2002.04 -

  • Affiliation:Kwansei Gakuin University
    Department:School of Science and Technology Department of Bioscience
    Title:Professor
    Date:2002.04 -

  • Department:工学研究科
    Title:Associate Professor (as old post name)
    Date:2001.08 - 2002.03

  • Department:工学研究科
    Title:Research Assistant
    Date:1994.04 - 2001.07

  • Department:医学部
    Title:Postdoctoral Fellow
    Date:1992.04 - 1994.03

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Association Memberships 【 display / non-display 】

  • Academic society name:日本生物工学会
    Academic country located:Japan

  • Academic society name:日本農芸化学会
    Academic country located:Japan

  • Academic society name:日本生化学会
    Academic country located:Japan

  • Academic society name:アメリカ微生物学会
    Academic country located:Japan

  • Academic society name:日本ポリアミン学会
    Academic country located:Japan

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Research Areas 【 display / non-display 】

  • Research field:Life Science / Applied microbiology

  • Research field:Manufacturing Technology (Mechanical Engineering, Electrical and Electronic Engineering, Chemical Engineering) / Biofunction and bioprocess engineering

  • Research field:Life Science / Molecular biology

  • Research field:Life Science / Applied microbiology

  • Research field:Life Science / Molecular biology

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Qualification acquired 【 display / non-display 】

  • Qualification title:High School Teacher Specialization License (first and second kind,specialization)

  • Qualification title:Junior High School Teacher Specialization License (first and second kind,specialization)

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Teacher organization examination execution 【 display / non-display 】

  • School name: Kwansei Gakuin University
    Career status: Professor
    Date of authorization: 2006.04

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Papers 【 display / non-display 】

  • Language: English
    Title: Branched-chain polyamines: evolutionary adaptation and biotechnological potential.
    Journal name: Amino Acids  vol.58  (p.17 - )
    Date of publication: 2026.02
    Author(s): Fujiwara, S. and Fukuda, W.

    DOI: https://doi.org/10.1007/s00726-026-03506-4
    Type of publication: Research paper (scientific journal)
    Field of experts:Life Science / Applied microbiology

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  • Language: English
    Title: Solid-state cultivation-specific agmatine production by Aspergillus oryzae: current understanding and perspectives.
    Journal name: Amino Acids  vol.58  (p.13 - )
    Date of publication: 2026.01
    Author(s): Akasaka, N., Watanabe, D., Yasukawa, K., and Fujiwara, S.

    DOI: https://doi.org/10.1007/s00726-026-03503-7
    Type of publication: Research paper (scientific journal)
    Field of experts:Life Science / Applied microbiology

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  • Language: English
    Title: Structure–function analysis and exonuclease deletion yield an improved strand-displacing DNA polymerase from Aeribacillus pallidus for efficient recombinase polymerase amplification.
    Journal name: J. Biotechnol.  vol.410  (p.321 - 330)
    Date of publication: 2026.01
    Author(s): Nishi, K., Inoue, E., Murakami, Y., Ishii, Y., Nakura, Y., Yanagihara, I., Yasukawa K. and Fujiwara, S.

    Type of publication: Research paper (scientific journal)
    Field of experts:Life Science / Applied microbiology

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  • Language: English
    Title: Importance of high temperature environment to maintain active form of arginine decarboxylase from hyperthermophilic archaeon Pyrobaculum calidifontis.
    Journal name: Biochem. Biophys. Res. Commun.  vol.786  (p.152705 - )
    Date of publication: 2025.09
    Author(s): Maekawa,K., Fujisaki,D., Ishii,Y., and Fujiwara,S.

    DOI: https://doi.org/10.1016/j.bbrc.2025.152705
    Type of publication: Research paper (scientific journal)
    Field of experts:Life Science / Applied microbiology, Life Science / Applied biochemistry

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  • Language: English
    Title: Replacement of branched-chain polyamine biosynthesis with thermospermine supports survival under both cold and heat stress in the hyperthermophilic archaeon <i>Thermococcus kodakarensis</i>
    Journal name: APPLIED AND ENVIRONMENTAL MICROBIOLOGY
    Date of publication: 2025.05
    Author(s): Fujiwara, S; Satake, R; Aoki, H; Yamada, K; Ishii, Y; Fukuda, W

    DOI: 10.1128/aem.00326-25
    Type of publication: Research paper (scientific journal)

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  • Fujiwara,S., Satake,R., Aoki,H., Yamada,K., Ishii,Y.,and Fukuda,W. Replacement of branched-chain polyamine biosynthesis with thermospermine supports survival under both cold and heat stress in the hyperthermophilic archaeon Thermococcus kodakarensis. Appl.Environ.Microbiol. 91, e00326-25 (American Society for Microbiology, Washington DC, USA) (https://doi.org/10.1128/aem.00326-25)

  • Fujiwara,S., Kawamori,E., Aoki,H., Nakura,Y., Ishii,Y., Masuo,S., Yasukawa,K., and Yanagihara,I. Branched-Chain Polyamine Beads for Sensitive Recovery and Detection of Low-Copy DNA in Aqueous Solutions J.Biotechnol. 405:290-298. (https://doi.org/10.1016/j.jbiotec.2025.06.008)

  • Maekawa,K., Fujisaki,D., Ishii,Y., and Fujiwara,S. Importance of high temperature environment to maintain active form of arginine decarboxylase from hyperthermophilic archaeon Pyrobaculum calidifontis. Biochem. Biophys. Res. Commun. (Elsevier Science B.V., Amsterdam, The Netherlands) 786 152705 (2025) (https://doi.org/10.1016/j.bbrc.2025.152705)

  • Nishi, K., Inoue, E., Murakami, Y., Ishii, Y., Nakura, Y., Yanagihara, I., Yasukawa K. and Fujiwara, S. Structure–function analysis and exonuclease deletion yield an improved strand-displacing DNA polymerase from Aeribacillus pallidus for efficient recombinase polymerase amplification. J. Biotechnol. 410:321-330 (2026) (https://doi.org/10.1016/j.jbiotec.2025.12.020))

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Books 【 display / non-display 】

  • Language: Japanese
    Title: 極限環境微生物の先端科学と社会実装最前線
    Publisher: NTS
    Date of publication: 2023.12
    Author(s): 藤原伸介

    Type of books: Scholarly book
    Area of responsibility: 超好熱菌の低温ストレス応答
    Authorship：Joint author

  • Language: English
    Title: Functional distribution of archaeal chaperonins. In Santosh Kumar, Shekhar Mande (ed), Prokaryotic Chaperonins
    Publisher: Springer Co.Ltd., New York
    Date of publication: 2017
    Author(s): Gao,L. and Fujiwara,S.

    Type of books: Scholarly book
    Area of responsibility: Functional distribution of archaeal chaperonins
    Authorship：Joint author

  • Language: English
    Title: Identification of branched-chain polyamines in hyperthermophiles. In Ruben Alcazar and Antonio Tiburcio (ed.) Polyamines: Methods and Protocols, Methods in Molecular Biology, vol. 1694:81-94
    Publisher: Springer Co.Ltd., New York
    Date of publication: 2017
    Author(s): Hidese,R., Fukuda,W., Niitsu,M., and Fujiwara, S.

    Type of books: Scholarly book
    Authorship：Joint author

  • Language: Japanese
    Title: 微生物のポリアミン　石野良純、跡見晴幸編 アーキアの生物学
    Publisher: 共立出版
    Date of publication: 2017
    Author(s): 藤原伸介

    Type of books: Scholarly book
    Authorship：Joint author

  • Language: English
    Title: Long-chain and branched polyamines in thermophilic microbes. In T. Kusano and H.Suzuki (ed.), Polyamines, a universal molecular nexus for growth, survival and specialised metabolism
    Publisher: Springer Co.Ltd., New York
    Date of publication: 2014
    Author(s): Fukuda, W., Hidese, R., and Fujiwara,S.

    Type of books: Scholarly book
    Area of responsibility: Long-chain and branched polyamines in thermophilic microbes.
    Authorship：Joint author

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MISC 【 display / non-display 】

  • Language：Japanese
    Title:「やはり対面交流は大切！」
    Journal name:ポリアミン学会誌  vol.8  (1)  (p.1 - 2)
    Date of publication:2022
    Author(s):藤原伸介

    Type of publication：Article, review, commentary, editorial, etc. (bulletin of university, research institution)

  • Language：English
    Title:The therapeutic and nutraceutical potential of agmatine, and its enhanced production using Aspergillus oryzae. Amino acids
    Journal name:Nature Springer  vol.52  (p.181 - 197)
    Date of publication:2020.04
    Author(s):Akasaka,N. and Fujiwara,S.

    Type of publication：Article, review, commentary, editorial, etc. (scientific journal)

  • Language：English
    Title:Alteration of enzymes and their application to nucleic acid amplification.
    Journal name:Int.J.Mol.Med.  vol.46  (p.1633 - 1643)
    Date of publication:2020
    Author(s):Yasukawa,K., Yanagihara,I., and Fujiwara,S.

    DOI:10.3892/ijmm.2020.4726
    Type of publication：Article, review, commentary, editorial, etc. (scientific journal)

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  • Language：Japanese
    Title:「働き方改革」に騙されてはいけない.
    Journal name:日本生物工学会誌  vol.98  (9)  (p.504 - 508)
    Date of publication:2020
    Author(s):藤原伸介

    Type of publication：Article, review, commentary, editorial, etc. (bulletin of university, research institution)

  • Language：Japanese
    Title:麹菌によるアグマチン高含有糖化液（甘酒）の開発
    Journal name:日本生物工学会誌  vol.97  (4)  (p.185 - 189)
    Date of publication:2019.04
    Author(s):赤坂直紀、藤原伸介

    Type of publication：Article, review, commentary, editorial, etc. (scientific journal)

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  • Akasaka, N., Watanabe, D., Yasukawa, K., and Fujiwara, S. Solid-state cultivation-specific agmatine production by Aspergillus oryzae: current understanding and perspectives. Amino Acids 58, 13 (2026) (https://doi.org/10.1007/s00726-026-03503-7)

  • Fujiwara, S. and Fukuda, W. Branched-chain polyamines: evolutionary adaptation and biotechnological potential. Amino Acids 58,17 (2026) (https://doi.org/10.1007/s00726-026-03506-4)

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Industrial Property Rights 【 display / non-display 】

  • Property type:Patent
    Title:ワンステップRT－PCR反応用組成物/キット
    Inventor(s)/Creator(s):藤原伸介

    Application number:特願2022-037964, 2022.03.11
    Publication number:特開2022-142759, 2022.09.30
    Country:Japan

  • Property type:Patent
    Title:活性型タンパク質の製造方法若しくはタンパク質の安定化方法、並びにそれらの方法のために用いられるポリペプチド、そのポリペプチドをコードするポリヌクレオチド、及びそのポリヌクレオチドを有するベクター
    Inventor(s)/Creator(s):藤原伸介，高楽

    Application number:特願2017-164319, 2017.08.29
    Country:Japan

  • Property type:Patent
    Title:昆虫誘引剤
    Inventor(s)/Creator(s):藤原伸介，合田慈子

    Application number:特願2017-035502, 2017.02.08
    Country:Japan

  • Property type:Patent
    Title:アグマチンの製造方法およびその利用
    Inventor(s)/Creator(s):藤原伸介,加藤彩織,他

    Application number:特願2016-242522, 2016.12.14
    Patent/Registration number:特許第6887663号, 2021.05.21
    Country:Japan

  • Property type:Patent
    Title:新規核酸合成法
    Inventor(s)/Creator(s):藤原伸介，藤原綾子，秀瀬涼太，他

    Application number:特願2016-190363, 2016.09.28
    Country:Japan

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Awards 【 display / non-display 】

  • Prize: 第３回アサヒビール生活科学賞
    Date awarded:2003.06
    Country:Japan
    Award type:Award from publisher, newspaper, foundation, etc.
    Awarding organization：アサヒビール学術振興財団　
    Award-winner (group):藤原　伸介

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Research Projects 【 display / non-display 】

  • Research category:Grant-in-Aid for Scientific Research(B)
    Project year：2021.04 - 2024.03
    Title:好熱菌における分岐鎖構造ポリアミンの作用機序

  • Research category:Grant-in-Aid for Challenging Research (Exploratory)
    Project year：2018.06 - 2020.03
    Title:耐熱性分子シャペロニンを利用したタンパク質の分画技術

  • Project year：2015.01 - 2015.12
    Title:ヘリカーゼを利用した高精度なDNA増幅技術（研究成果展開事業　研究成果最適展開支援プログラム　A-STEP探索タイプ）
    Awarding organization：独立行政法人科学技術振興機構

  • Project year：2014.12 - 2018.03
    Title:高感度かつ高精度な転写産物の検出技術の開発（先端計測分析技術・機器開発プログラム）
    Awarding organization：独立行政法人科学技術振興機構

  • Research category:Grant-in-Aid for Scientific Research(B)
    Project year：2014.04 - 2017.03
    Title:超好熱菌の低温適応機構に関する包括的理解　（研究課題番号：26292045）

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Presentations 【 display / non-display 】

  • Language:Japanese
    Conference name:第76回日本生物工学会大会
    International/Domestic presentation:Domestic presentation
    Holding date：2024.09
    Title:Aeribacillus pallidus H1 由来鎖置換型DNAポリメラーゼの安定化(2024年9月8日-10日)第76回日本生物工学会大会
    Presentation type:Oral presentation (general)

  • Language:Japanese
    Conference name:第76回日本生物工学会大会
    International/Domestic presentation:Domestic presentation
    Holding date：2024.09
    Title: Thermococcus kodakarensis 由来の2 種類の耐熱性ヘリカーゼを利用したヘリカーゼ依存型DNA 増幅法の試み(2024年9月8日-10日)第76回日本生物工学会大会（東京工業大学大岡山キャンパス
    Presentation type:Oral presentation (general)

  • Language:English
    Conference name:International Conference on the Biological Roles of Polyamines 2024, 7th Yamada Symposium
    International/Domestic presentation:International presentation
    Holding date：2024.08
    Title: Exploring Novel Thermospermine Oxidase in Thermophilic Archaeon Pyrobaculum calidifontis.
    Presentation type:Poster presentation

  • Language:English
    Conference name:International Conference on the Biological Roles of Polyamines 2024, 7th Yamada Symposium
    International/Domestic presentation:International presentation
    Holding date：2024.08
    Title: Biological Role of Branched-chain Polyamines in Survival of Hyperthermophiles under Extremal Conditions.
    Presentation type:Oral presentation (general)

  • Language:English
    Conference name:International Conference on the Biological Roles of Polyamines 2024, 7th Yamada Symposium
    International/Domestic presentation:International presentation
    Holding date：2024.08
    Title: Enzymatic Characteristics of Aminopropyltransferases in Hyperthermophilic Microorganisms: Comparative Analysis of Branched Chain Polyamine and Norspermine Synthesis.
    Presentation type:Oral presentation (general)

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Teaching Experience (Off-campus) 【 display / non-display 】

  • Subject：卒業実験及び演習
    Institution name：Kwansei Gakuin University

  • Subject：外国書講読
    Institution name：Kwansei Gakuin University

  • Subject：研究計画法IIＢ
    Institution name：Kwansei Gakuin University

  • Subject：研究計画法IIＡ
    Institution name：Kwansei Gakuin University

  • Subject：生命科学入門実験
    Institution name：Kwansei Gakuin University

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Committee Memberships 【 display / non-display 】

  • Organization name:極限環境生物学会
    Committee name:評議員

  • Organization name:極限環境生物学会
    Committee name:評議員

  • Organization name:日本ポリアミン学会
    Committee name:評議員

  • Organization name:日本ポリアミン学会
    Committee name:評議員

  • Organization name:日本農芸化学会
    Committee name:関西支部参与

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Social Contribution 【 display / non-display 】

  • Title:創発的研究支援事業　事前評価

    Description:事前評価における外部専門家

  • Title:創発的研究支援事業 事前評価

    Description:事前評価における外部専門家

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